Which Dna Genome, Upon Entry Into the Cell, Can Be Immediately Copied Into Mrna?
New Results
SARS-CoV-2 RNA reverse-transcribed and integrated into the human being genome
Summary
Prolonged SARS-CoV-ii RNA shedding and recurrence of PCR-positive tests have been widely reported in patients after recovery, even so these patients about ordinarily are non-infectiousone–14. Here we investigated the possibility that SARS-CoV-two RNAs can be opposite-transcribed and integrated into the human genome and that transcription of the integrated sequences might account for PCR-positive tests. In back up of this hypothesis, nosotros found chimeric transcripts consisting of viral fused to cellular sequences in published data sets of SARS-CoV-two infected cultured cells and chief cells of patients, consistent with the transcription of viral sequences integrated into the genome. To experimentally corroborate the possibility of viral retro-integration, we describe show that SARS-CoV-2 RNAs tin can exist reverse transcribed in man cells by opposite transcriptase (RT) from LINE-ane elements or by HIV-1 RT, and that these DNA sequences can exist integrated into the cell genome and subsequently exist transcribed. Homo endogenous LINE-i expression was induced upon SARS-CoV-2 infection or past cytokine exposure in cultured cells, suggesting a molecular mechanism for SARS-CoV-2 retro-integration in patients. This novel feature of SARS-CoV-2 infection may explain why patients can continue to produce viral RNA later recovery and suggests a new aspect of RNA virus replication.
Competing Interest Statement
The authors take declared no competing involvement.
Copyright
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Source: https://www.biorxiv.org/content/10.1101/2020.12.12.422516v1
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